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Given below are a few statements on technologies/concepts related to development of transgenic plants:

A. Frequency of genetic transformation is influenced only by the genes of Agrobacterium and not by those of the host plants.

B. Transgenic plants containing a single copy of the transgene are preferred over those that contain multiple transgene copies for subsequent genetic analysis.

C. Supervirulent strains of Agrobacterium can be generated by increasing the copy number of virulence genes.

D. A nonconditional negative selection marker has to be necessarily used with a strong constitutive promoter for the development of transgenic plants.

Which one of the following options represents a combination of all INCORRECT statements?

This question was previously asked in
CSIR-UGC (NET) Life Science: Held on (17 Feb 2022 Shift 1)
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  1. A and D only
  2. C only
  3. B and C only
  4. D only

Answer (Detailed Solution Below)

Option 1 : A and D only
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Detailed Solution

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The correct answer is Option 1 i.e. A and D only

Concept:

  • Agrobacterium-mediated gene transfer is known to create marker-free plants, which are necessary for the commercialization of transgenic crops, and integrates foreign genes mostly at a single locus in the host plant without an associated vector backbone.

Overview:

F1 Teaching Arbaz 02-06-2023 Moumita D16

Explanation:

Statement A:- INCORRECT

  • The frequency of genetic transformation is influenced by both the genes of Agrobacterium and by those of the host plants.

Statement B:- CORRECT

  • High-copy number transgenes have been linked to functional and structural instability.

Statement C:- CORRECT

  • Supervirulent strains of Agrobacterium can be generated by increasing the copy number of virulence genes.

Statement D:- INCORRECT

  • A nonconditional negative selection marker need not be necessarily used with a strong constitutive promoter for the development of transgenic plants.

 Therefore, the correct Option is A ( A and D only).

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